Purification and the effects on structure and bioactivity for polysaccharide from Actinidia valvata Dunn. using macroporous adsorption resin

Resin AB-8 was screened from seven resins with different polarity and particle size because of its better decoloration deproteinization efficiency to polysaccharide the root Actinidia valvata (AvPs), then optimized parameter conditions were applied by single-factor experiments shown as follows: a concentration crude 3 mg/mL, an adsorption time 40 min, initial pH 7.0, flow rate 1.5 BV/h. In addition, results HPGPC, FT-IR, PMP-HPLC-DAD, 1H NMR Congo red test showed that sugar chain, structural group, monosaccharide composition triple-helix conformation structure AvPs not broken during resin treatment. Moreover, could keep stability biological activity after treatment, revealed obviously antioxidant anticancer activities in vitro well. This research suggests this method is promising for decolorization which be explored potential or agent use functional food medicine.